Gene structure for the. alpha. 1 chain of a human short-chain collagen (type XIII) with alternatively spliced transcripts and translation termination codon at the 5' end of the last exon
- Univ. of Oulu (Finland)
Two overlapping human genomic clones that encode a short-chain collagen, designated {alpha}1(XIII), were isolated by using recently described cDNA clones. Characterization of the cosmid clones that span {approx} 65,000 base pairs (bp) of the 3' end of the gene established several unusual features of this collagen gene. The last exon encodes solely the 3' untranslated region and it begins with a complete stop codon. The 10 adjacent exons vary in size from 27 to 87 bp and two of them are 54 bp. Therefore, the {alpha}1-chain gene of type XIII collagen has some features found in genes for fibrillar collagens but other features that are distinctly different. Previous analysis of overlapping cDNA clones and nuclease S1 mapping of mRNAs indicated one alternative splicing site causing a deletion of 36 bp from the mature mRNA. The present study showed that the 36 bp is contained within the gene as a single exon and also that the gene has a 45-bp -Gly-Xaa-Xaa- repeat coding exon not found in the cDNA clones previously characterized. Nuclease S1 mapping experiments indicated that this 45-bp exon is found in normal human skin fibroblast mRNAs. Accordingly, the data demonstrate that there is alternative splicing of at least two exons of the type {alpha}1(XIII)-chain gene.
- OSTI ID:
- 5607153
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:20; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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COLLAGEN
AMINO ACID SEQUENCE
GENES
MOLECULAR STRUCTURE
RECOMBINANT DNA
DNA SEQUENCING
CODONS
FIBROBLASTS
MAN
MESSENGER-RNA
SKIN
TRANSCRIPTION
ANIMAL CELLS
ANIMALS
BODY
CONNECTIVE TISSUE CELLS
DNA
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RNA
SCLEROPROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
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550201* - Biochemistry- Tracer Techniques