Polymorphism of the membrane proteinases of the mitochondria
Journal Article
·
· Biochemistry (Engl. Transl.); (United States)
OSTI ID:5767865
Three protein fractions capable of catalyzing the proteolysis of cytochrome c and three other fractions catalyzing the hydrolysis of N-..cap alpha..-benzoyl-L-arginine-p-nitroanilide (BAPA) and N-..cap alpha..-benzoyl-L-arginine-..beta..-naphthylamide (BANA) were separated by electrophoresis in polyacrylamide gel in the absence of SDS detergent extracts from ultrasonic submitochondrial particles (SMP). The indicated fractions were isolated from gel and studied according to a series of parameters. It was shown that cytochrome c hydrolases have the same molecular weight (17,000) but different isoelectric points (4.0, 4.2, and 4.4). The total cytochrome c hydrolase activity of these enzymes was inhibited by phenylmethylsulfonyl fluoride but was insensitive to ethylenediaminetetraacetate and o-phenanthroline. The three BANA (BAPA) hydrolases also had similar molecular weights (approx. 17,500) and different isoelectric points (4.2, 4.3, and 4.7). In addition to the indicated hydrolases, minor components, possessing the same activities but differing in strength of the bond to the inner mitochondrial membrane, molecular weight, and sensitivity to proteinase inhibitors, were detected in the detergent extracts of the SMP. It was concluded that there is a polymorphism of the proteinases associated with the inner mitochondrial membrane.
- Research Organization:
- M.V. Lomonosov Moscow State Univ., USSR
- OSTI ID:
- 5767865
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:3; ISSN BIORA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
CATALYTIC EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CYTOCHROMES
DECOMPOSITION
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
FUNGI
HYDROLASES
HYDROLYSIS
LABELLED COMPOUNDS
LYSIS
MEMBRANES
MICROORGANISMS
MITOCHONDRIA
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PIGMENTS
PLANTS
PROTEINS
PROTEOLYSIS
RADIOENZYMATIC ASSAY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SOLVOLYSIS
TRITIUM COMPOUNDS
YEASTS
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
CATALYTIC EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CYTOCHROMES
DECOMPOSITION
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
FUNGI
HYDROLASES
HYDROLYSIS
LABELLED COMPOUNDS
LYSIS
MEMBRANES
MICROORGANISMS
MITOCHONDRIA
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PIGMENTS
PLANTS
PROTEINS
PROTEOLYSIS
RADIOENZYMATIC ASSAY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SOLVOLYSIS
TRITIUM COMPOUNDS
YEASTS