Purification and some properties of two proteinases from Crotalus adamanteus venom that inactivate human. cap alpha. /sub 1/-proteinase inhibitor

Journal Article · Sat Nov 25 04:00:00 EST 1978 · J. Biol. Chem.; (United States)

OSTI ID:6594215

Kurecki, T; Laskowski, M Sr; Kress, L F

Two proteinases (proteinases I and II) have been purified from Crotalus adamanteus venom to the stage of electrophoretic homogeneity and proteinase II has been crystallized. The proteinases differ slightly in molecular weight and amino acid composition. Both are metalloenzymes requiring Zn/sup 2 +/ or Ca/sup 2 +/, or both; neither requires thiol compounds for activation. The proteinases are free of esterolytic activity against benzoyl-L-arginine ethyl ester and benzoyl-L-tyrosine ethyl ester. Proteinase II cleaves the oxidized B chain of insulin at the bonds Phe/sub 1/--Val/sub 2/, His/sub 5/--Leu/sub 6/, His/sub 10/--Leu/sub 11/, Ala/sub 14/--Leu/sub 15/, Leu/sub 15/--Tyr/sub 16/, and Tyr/sub 16/--Leu/sub 17/. Digestion of polylysine and polyarginine by proteinase II liberates products ranging from dodecapeptides to hexapeptides. Proteinases I and II catalytically inactivate human plasma ..cap alpha../sub 1/--proteinase inhibitor (54,000 daltons). Electrophoretic analysis of the reaction of proteinase II with ..cap alpha../sub 1/--proteinase inhibitor reveals that an inactivated inhibitor species of 50,000 daltons is formed, and a peptide of 4,000 daltons is released. The gradual disappearance of the native inhibitor results in the corresponding loss of inhibitory activity against trypsin and chymotrypsin.

• Research Organization: Roswell Park Memorial Inst., Buffalo, NY
• OSTI ID: 6594215
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 253:22; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATOMIC IONS
CALCIUM IONS
CHARGED PARTICLES
CHEMICAL ANALYSIS
CHEMICAL PROPERTIES
ENZYME INHIBITORS
ENZYMES
HORMONES
HYDROLASES
INACTIVATION
INSULIN
IONS
METABOLISM
MOLECULAR WEIGHT
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PURIFICATION
REPTILES
SNAKES
VENOMS
VERTEBRATES
ZINC IONS