Effect of intracellular pH on the rate of chloride uptake and efflux in different mammalian cell lines
The effect of the internal pH on the rate of /sup 36/Cl/sup -/ uptake and efflux was measured. While the rate of /sup 36/Cl/sup -/ uptake by the antiport increased almost 10-fold over a narrow pH range in a number of cell lines, in other cells the pH-induced increase was considerably less. With increasing pH, the rate of /sup 36/Cl/sup -/ efflux into chloride-free buffers increased strongly over a narrow pH range in all cell lines studied. Two groups of cell lines appeared, one group where the half-maximal increase in uptake and efflux rate was at pH 7.0-7.1 and another group where the corresponding values were 0.2-0.4 pH unit higher. The stoichiometry between chloride influx and chloride-stimulated efflux was close to 1:1 under various conditions.
- Research Organization:
- Norwegian Cancer Society, Oslo
- OSTI ID:
- 5767842
- Journal Information:
- Biochemistry; (United States), Vol. 26:10
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHLORIDES
MEMBRANE TRANSPORT
CHLORINE 36
ANIMAL CELLS
HELA CELLS
HYDROCHLORIC ACID
KIDNEYS
MAMMARY GLANDS
MAN
PH VALUE
PHYSIOLOGY
STOICHIOMETRY
TUMOR CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHLORINE COMPOUNDS
CHLORINE ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
INORGANIC ACIDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANS
PRIMATES
RADIOISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550301* - Cytology- Tracer Techniques