Effect of potassium depletion of Hep 2 cells on intracellular pH and on chloride uptake by anion antiport

Journal Article · Wed Apr 01 04:00:00 EST 1987 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041310103· OSTI ID:6498878

Madshus, I H; Tonnessen, T I; Olsnes, S; Sandvig, K

The effect of K+ depletion of Hep 2 cells on ion fluxes, internal pH, cell volume, and membrane potential was studied. The cells were depleted of K+ by incubation in K+-free buffer with or without a preceding exposure to hypotonic medium. Efflux of K+ in cells not exposed to hypotonic medium was inhibited by furosemide or by incubation in Na+-free medium, indicating that in this case at least part of the K+ efflux occurs by Na+/K+/Cl- cotransport. After exposure to hypotonic medium, K+ efflux was not inhibited by furosemide, whereas it was partly inhibited by 4,4'-diisothiocyano-2,2'-stilbene-disulfonic acid (DIDS). Exposure to hypotonic medium induced acidification of the cytosol, apparently because of efflux of protons from intracellular acidic vesicles. When isotonicity was restored, a rebound alkalinization of the cytosol was induced, because of activation of the Na+/H+ antiporter. While hypotonic shock and a subsequent incubation in K+-free buffer rapidly depolarized the cells, depolarization occurred much more slowly when the K+ depletion was carried out by incubation in K+-free buffer alone. The cell volume was reduced in both cases. K+ depletion by either method strongly reduced the ability of the cells to accumulate /sup 36/Cl- by anion antiport, and K+-depleted cells were unable to increase the rate of /sup 36/Cl- uptake in response to alkalinization of the cytosol.

• Research Organization: Institute for Cancer Research at the Norweigian Radium Hospital, Oslo, Norway
• OSTI ID: 6498878
• Journal Information: J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 1; ISSN JCLLA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
ANIONS
BARYONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHLORIDES
CHLORINE 36
CHLORINE COMPOUNDS
CHLORINE ISOTOPES
DEPOLARIZATION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
FUNCTIONS
HADRONS
HALIDES
HALOGEN COMPOUNDS
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANE TRANSPORT
MEMBRANES
METALS
NUCLEI
NUCLEONS
ODD-ODD NUCLEI
PH VALUE
POTASSIUM
PROTONS
RADIOISOTOPES
TRACER TECHNIQUES
YEARS LIVING RADIOISOTOPES