Biosynthesis of thiamine triphosphate and identification of thiamine diphosphate-binding proteins of rat liver hyaloplasm

Journal Article · Mon Mar 10 04:00:00 EST 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5767682

Voskoboev, A I; Chernikevich, I P

The nature of the proteins of rat liver hyaloplasm that bind ThDP* was studied. When injection of (/sup 14/C)thiamine was used as the marker, an electrophoretically homogeneous protein preparations, containing (/sup 14/C)ThDP, identified as transketolase, was isolated from the soluble fraction of the liver. No other nonenzymatic proteins that bind ThDP and might serve as the substrate in the synthesis of ThTP were detected in the hyaloplasm. It was shown that transfer of the phosphate group by rat liver ThDP kinase occurs to the free ThDP, and not to protein-bound ThDP, as was previously asserted.

• OSTI ID: 5767682
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:9; ISSN BIORA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
AZINES
AZOLES
BIOCHEMISTRY
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CATALYTIC EFFECTS
CELL CONSTITUENTS
CHEMISTRY
COENZYMES
CYTOPLASM
DIGESTIVE SYSTEM
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LIVER
LIVER CELLS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOSPHORUS COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
PYRIMIDINES
RADIOENZYMATIC ASSAY
RADIORECEPTOR ASSAY
RATS
RODENTS
SOMATIC CELLS
SYNTHESIS
THIAMINE
THIAZOLES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
VITAMIN B GROUP
VITAMINS