Coenzyme metabolism in rat liver transketolase
Journal Article
·
· Biochemistry (Engl. Transl.); (United States)
OSTI ID:5721633
On the basis of the results of kinetic investigations, two binding sites for hydroxythiamine diphosphate were determined in apotransketolase, with sharply differing values of K/sub i/: (7-22) x 10/sup -9/ and (13.0-19.7) x 10/sup -8/ M. A study was made of the turnover rate of thiamine diphosphate in holotransketolase in rat liver tissue by a radioisotope method, using (/sup 14/C) thiamine as the labeled precursor. The half-substitution time and rate constant of degradation of the coenzyme in transketolase are close in absolute values to the analogous indices for the protein portion of the enzyme and constitute 153 h and 0.108 day/sup -1/, respectively. Rat liver transketolase exists in vivo in the form of a substituted ..cap alpha..-carbanion. Replacement of thiamine diphosphate by hydroxythiamine diphosphate in the holoenzyme has no effect on the formation of the intermediate ..cap alpha..-carbanion form of the enzyme.
- OSTI ID:
- 5721633
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:7; ISSN BIORA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AZINES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIODEGRADATION
BIOLOGICAL PATHWAYS
BODY
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHEMISTRY
COENZYMES
DECOMPOSITION
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LABELLING
LIVER
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PYRIMIDINES
RADIOENZYMATIC ASSAY
RATS
REACTION KINETICS
RODENTS
THIAMINE
THIAZOLES
TRANSFERASES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AZINES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIODEGRADATION
BIOLOGICAL PATHWAYS
BODY
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHEMISTRY
COENZYMES
DECOMPOSITION
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LABELLING
LIVER
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
PYRIMIDINES
RADIOENZYMATIC ASSAY
RATS
REACTION KINETICS
RODENTS
THIAMINE
THIAZOLES
TRANSFERASES
VERTEBRATES
VITAMIN B GROUP
VITAMINS