Effects of mutation on the downfield proton nuclear magnetic resonance spectrum of the 5S RNA of Escherichia coli

Journal Article · Tue Sep 08 04:00:00 EDT 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00392a012· OSTI ID:5767292

Gewirth, D T; Moore, P B

The imino proton spectra of several mutants of the 5S RNA of Escherichia coli are compared with that of the wild type. Three of the variants discussed are point mutations, and the fourth is a deletion mutant lacking bases 11-69 of the parent sequence, all obtained by site-directed mutagenesis techniques. The spectroscopic effects of mutation are limited in all cases, and the differences between normal and mutant spectra can be used to make or confirm the assignments of resonances. Several new assignments in the 5S spectrum are reported. Spectroscopic differences due to sequence differences permit the products of single genes within the 5S gene family to be distinguished and their fates followed by NMR.

• Research Organization: Yale Univ., New Haven, CT
• OSTI ID: 5767292
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:18; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BACTERIA
BARYONS
ELEMENTARY PARTICLES
ESCHERICHIA COLI
FERMIONS
GENE MUTATIONS
HADRONS
MAGNETIC RESONANCE
MICROORGANISMS
MUTAGENESIS
MUTANTS
MUTATIONS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
PROTONS
RESONANCE
RIBOSOMAL RNA
RNA
SPECTRA
STRUCTURAL CHEMICAL ANALYSIS