Studies of the helix V-loop E domain in the 5S RNA of Escherichia coli by sup 1 H and sup 31 P NMR

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6159039

Zhang, Penghua

Complete assignments of the imino proton resonances of 5S RNA fragment 1 and fragment 1/L25 complex were made based upon NM spectra collected from samples of fragment 1 and fragment 1/L25 complex segmentally labelled with {sup 15}N, as well as upon data from mutant 5S RNAs and from nuclear Overhauser effect studies. These assignments provided useful information about a special structural domain in 5S RNA, helix V-loop E (see Figure 1-1) and about the effects of L2 binding on that region. Using a series of selectively deuterated fragment 1 samples, three NOE relationships between imino and aromatic proton resonances in the helix V-loop E domain were characterized as to strand and proton types. These results show that this domain exists in a duplex form although it is not a regular double helix. {sup 31}P NMR spectra of 5S and several derivatives show only a small number of resolved, single phosphorus, phosphodiester resonances, which indicate that 5S RNA does not have a tightly folded, tRNA like, tertiary structure. The resolved resonances in the 5S spectrum were assigned to phosphorus atoms in loops D and E. These studies also suggested that the helix V-loop E domain exists in more than one conformation.

• Research Organization: Yale Univ., New Haven, CT (USA)
• OSTI ID: 6159039
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NITROGEN 15
NITROGEN ISOTOPES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
RESONANCE
RNA
SPECTRA
STABLE ISOTOPES
TRACER TECHNIQUES