Mutation induction in repair-deficient strains of Drosophila
Experimental evidence indicates a polygenic control of mutagenesis in Drosophila melanogaster. In oocytes chromosome aberrations detected as half-translocations or dominant lethals depend on a repair system which in a number of genetically nonrelated strains shows different repair capacities. Sister chromatid exchanges are easily studied as ring chromosome losses. They develop through a genotype controlled mechanism from, premutational lesions. Stocks with particular pairs of third chromosomes were discovered in which increased sensitivity of larvae to the toxic effects of a monofunctional alkylating agent correlates with high frequencies of x-ray induced SCE's. Sex-linked mutagen-sensitive mutants could be shown to control mutation fixation: pronounced maternal effects were found when sperm carrying particular types of premutational lesions were introduced into different types of mutant oocytes. The mutant mus(1)101D1 was found to be unable to process lesions induced by the crosslinking agent nitrogen mustard into point mutations. Alkylation damage leads to increased point mutation frequencies in the excision repair deficient mutant mei-9L1, but to reduced frequencies in the post-replication repair deficient mutant mei-41D5. It became clear that the study of maternal effects on mutagenized sperm represents an efficient tool to analyze the gentic control of mutagenesis in the eukaryotic genome of Drosophila melanogaster.
- OSTI ID:
- 5764744
- Journal Information:
- Basic Life Sci.; (United States), Vol. 15
- Country of Publication:
- United States
- Language:
- English
Similar Records
mei-9/sup a/ mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair. [UV radiation, MMS, nitrogen mustard, caffeine]
Isolation and characterization of X-linked mutants of Drosophila melanogaster which are sensitive to mutagens. [UV and gamma radiation, MMS, nitrogen mustard]
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHROMOSOMAL ABERRATIONS
BIOLOGICAL REPAIR
MUTAGENESIS
BIOCHEMICAL REACTION KINETICS
ALKYLATING AGENTS
DNA
DROSOPHILA
GENETIC RADIATION EFFECTS
MUTANTS
MUTATION FREQUENCY
OOCYTES
SISTER CHROMATID EXCHANGES
X RADIATION
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
DIPTERA
ELECTROMAGNETIC RADIATION
FLIES
FRUIT FLIES
GENETIC EFFECTS
GERM CELLS
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
KINETICS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
550400* - Genetics
560114 - Radiation Effects on Biochemicals- In Animals- (-1987)