Capillary array electrophoresis using laser-excited confocal fluorescence detection
- Univ. of California, Berkeley, CA (United States)
Capillary electrophoresis (CE) has found widespread application in analytical and biomedical research, and the scope and sophistication of CE is still rapidly advancing. Gel-filled capillaries have been employed for the rapid separation and analysis of synthetic polynucleotides, DNA sequencing fragments, and DNA restriction fragments. Open-tube capillary electrophoresis has attained subattomole detection levels in amino acid separations 14 and proven its utility for the separation of proteins, viruses, and bacteria. Separation of the optical isomers of dansyl amino acids has also been successfully demonstrated. Micellar electrokinetic capillary chromatography, isoelectric focusing, and on-column derivatization can all be performed on CE columns, demonstrating the utility of capillary electrophoresis as an analytical and micropreparative tool. 29 refs., 6 figs., 1 tab.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 576402
- Journal Information:
- Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 8 Vol. 64; ISSN 0003-2700; ISSN ANCHAM
- Country of Publication:
- United States
- Language:
- English
Similar Records
Combined capillary electrophoresis and electrospray ionization mass spectrometry
A new post-column reactor-laser induced fluorescence detector for capillary electrophoresis