Cloning and expression of a rat brain GABA transporter

Journal Article · Fri Sep 14 04:00:00 EDT 1990 · Science (Washington, D.C.); (USA)

DOI:https://doi.org/10.1126/science.1975955· OSTI ID:5763143

Guastella, J; Czyzyk, L; Davidson, N; Lester, H A ^[1]; Nelson, N; Nelson, H; Miedel, M C ^[2]; Keynan, S; Kanner, B I ^[3]

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1. California Institute of Technology, Pasadena, CA (USA)
2. Roche Institute of Molecular Biology, Nutley, NJ (USA)
3. Hebrew Univ., Jerusalem (Israel)

A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter {gamma}-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated ({sup 3}H)GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium- and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.

• OSTI ID: 5763143
• Journal Information: Science (Washington, D.C.); (USA), Journal Name: Science (Washington, D.C.); (USA) Vol. 249:4974; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
AMINOBUTYRIC ACID
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
BRAIN
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHEMICAL PROPERTIES
CLONING
DNA HYBRIDIZATION
DNA-CLONING
DRUGS
FROGS
GENE REGULATION
GERM CELLS
HYBRIDIZATION
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
MESSENGER-RNA
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEIC ACIDS
OOCYTES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RATS
RNA
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES