Cloning of the cocaine-sensitive bovine dopamine transporter

Journal Article · Sun Dec 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.24.11168· OSTI ID:5703837

Usdin, T B; Chen, C; Brownstein, M J; Hoffman, B J ^[1]; Mezey, E ^[2]

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1. National Inst. of Mental Health, Bethesda, MD (United States)
2. Semmelweis Univ., Budapest (Hungary)

A cDNA encoding the dopamine transporter from bovine brain substantia nigra was identified on the basis of its structural homology to other, recently cloned, neurotransmitter transporters. The sequence of the 693-amino acid protein is quite similar to those of the rat {gamma}-aminobutyric acid, human norepinephrine, and rat serotonin transporters. Dopamine transporter mRNA was detected by in situ hybridization in the substantia nigra but not in the locus coeruleus, raphe, caudate, or other brain areas. ({sup 3}H)Dopamine accumulation in tissue culture cells transfected with the cDNA was inhibited by amphetamine, cocaine, and specific inhibitors of dopamine transports, including GBR12909.

• OSTI ID: 5703837
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:24; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COCAINE
BIOLOGICAL EFFECTS
DOPAMINE
MEMBRANE TRANSPORT
MEMBRANE PROTEINS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
CELL CULTURES
DNA-CLONING
NEUROREGULATORS
TRITIUM COMPOUNDS
ALKALOIDS
AMINES
ANESTHETICS
ANTIDEPRESSANTS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM AGENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CLONING
DNA
DNA HYBRIDIZATION
DRUGS
HYBRIDIZATION
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
PROTEINS
PSYCHOTROPIC DRUGS
STRUCTURAL CHEMICAL ANALYSIS
SYMPATHOMIMETICS
550200* - Biochemistry