Post-UV colony-forming ability of normal fibroblast strains and of the xeroderma pigmentosum group G strain

Journal Article · Thu Jan 01 04:00:00 EST 1981 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12524886· OSTI ID:5762797

Barrett, S F; Tarone, R E; Moshell, A N; Ganges, M B; Robbins, J H

In xeroderma pigmentosum, an inherited disorder of defective DNA repair, post-uv colony-forming ability of fibroblasts from patients in complementation groups A through F correlates with the patients' neurological status. The first xeroderma pigmentosum patient assigned to the recently discovered group G had the neurological abnormalities of XP. Researchers have determined the post-uv colony-forming ability of cultured fibroblasts from this patient and from 5 more control donors. Log-phase fibroblasts were irradiated with 254 nm uv light from a germicidal lamp, trypsinized, and replated at known densities. After 2 to 4 weeks' incubation the cells were fixed, stained and scored for colony formation. The strains' post-uv colony-forming ability curves were obtained by plotting the log of the percent remaining post-uv colony-forming ability as a function of the uv dose. The post-uv colony-forming ability of 2 of the 5 new normal strains was in the previously defined control donor zone, but that of the other 3 extended down to the level of the most resistant xeroderma pigmentosum strain. The post-uv colony-forming ability curve of the group G fibroblasts was not significantly different from the curves of the group D fibroblast strains from patients with clinical histories similar to that of the group G patient.

• OSTI ID: 5762797
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 76:1; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM