In vitro gamma irradiation Medical Center of leukemic cells in mice, rats, and guinea pigs

Journal Article · Mon Dec 01 04:00:00 EST 1980 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.77.12.7435· OSTI ID:5762775

Gross, L; Dreyfuss, Y; Ehrenreich, T; Feldman, D; Limbert, L M

In vitro gamma irradiation of virus-induced (Gross) mouse leukemia cells at doses of 350 to 1600 rads (1 rad = 0.01 gray) had no effect on their ability to induce leukemia, usually within 2 weeks, after transplantation into syngeneic mice. However, when cells irradiated at doses of 2000-20,000 rads were transplanted, they induced leukemia after a latency period exceeding 2.5 months, similar to the results observed in mice inoculated with filtered mouse leukemia extracts. Similar results were also obtained after irradiation of leukemic cells derived from rats in which leukemia had been induced by rat-adapted mouse leukemia virus. Apparently, gamma irradiation at a dose of, or exceeding, 2000 rads, inhibits the ability of mouse and rat leukemic cells to induce leukemia after transplantation into syngeneic hosts; however, it does not inactivate the virus carried by such cells nor prevent it from inducing leukemia. (In previous experiments, doses of more than 4,500,000 rads were needed to inactivate the passage A (Gross) leukemia virus carried in either mouse or rat leukemic cells.) In vitro gamma irradiation of L2C guinea pig leukemic cells at doses of 750 to 2500 rads had no apparent effect on their ability to induce leukemia after transplantation into strain 2 guinea pigs. However, irradiation at doses of 3250 to 20,000 rads inactivated their ability to do so. The morphology of mouse, rat, and guinea pig leukemic cells and the virus particles present in such cells was not affected by irradiation at doses of 20,000 rads.

• Research Organization: Veterans Administration, Bronx, NY
• OSTI ID: 5762775
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 77:12; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHEMISTRY
DISEASES
DOSES
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GUINEA PIGS
HEMIC DISEASES
IN VITRO
IONIZING RADIATIONS
LEUKEMIA
LEUKEMIA VIRUSES
MAMMALS
MICE
MICROORGANISMS
MORPHOLOGY
NEOPLASMS
ONCOGENIC VIRUSES
PARASITES
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
TRANSPLANTS
VERTEBRATES
VIRUSES