Ultraviolet light induction of diphtheria toxin-resistant mutations in normal and DNA repair-deficient human and Chinese hamster fibroblasts

Journal Article · Tue Jan 01 00:00:00 EST 1980 · Basic Life Sci.; (United States)

DOI:https://doi.org/10.1007/978-1-4684-3842-0_21· OSTI ID:5762749

Trosko, J E; Schultz, R S; Chang, C C; Glover, T

The role on unrepaired DNA lesions in the production of mutations is suspected of contributing to the initiation phase of carcinogenesis. Since the molecular basis of mutagenesis is not understood in eukaryotic cells, development of new genetic markers for quantitative in vitro measurement of mutations for mammalian cells is needed. Furthermore, mammalian cells, genetically deficient for various DNA repair enzymes, will be needed to study the role of unrepaired DNA lesions in mutagenesis. The results in this report relate to preliminary attempts to characterize the diphtheria toxin resistance marker as a useful quantitative genetic marker in human cells and to isolate and characterize various DNA repair-deficient Chinese hamster cells.

• OSTI ID: 5762749
• Journal Information: Basic Life Sci.; (United States), Vol. 15
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DNA
BIOLOGICAL REPAIR
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
COMPARATIVE EVALUATIONS
DIPHTHERIA
DOSE-RESPONSE RELATIONSHIPS
HAMSTERS
MAN
MUTAGENESIS
QUANTITATIVE CHEMICAL ANALYSIS
SURVIVAL CURVES
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BACTERIAL DISEASES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CHEMICAL ANALYSIS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
INFECTIOUS DISEASES
MAMMALS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550200 - Biochemistry