The mutation studies of mutagen-sensitive and DNA repair mutants of Chinese hamster fibroblasts
Journal Article
·
· Environ. Mutagen.; (United States)
We have previously reported the isolation and partial characterization of DNA repair and/or mutagen-sensitive mutant Chinese hamster cell strains. Here we present the results of a detailed study of the ultraviolet light (UV)-induced mutability of one of these strains, UVs-7, and provide preliminary mutability data on two additional lines, UVr-23 and UVs-40. UVs-7 in extremely deficient in unscheduled DNA synthesis (UDS) but only slightly more sensitive to UV than the parental line. When examined for the UV-inducibility of mutants resistant to ouabain, 6-thioguanine, or diphtheria toxin, UVs-7 was found to be hypermutable at all three loci as compared to the parental line. The degree of hypermutability was not the same for any two loci. UVs-40, a highly UV-sensitive strain, was also found to be hypermutable at the ouabain-resistant (ouar) locus. UVr-23, which is UV-resistant and more proficient at UDS than the parental line, appeared to exhibit a tendency toward hypomutability at both the ouabain(ouar) and 6-thioguanine--resistant (6TGr) loci. Further characterization of all these lines should aid in delineating mammalian mechanisms of DNA repair and mutagenesis.
- OSTI ID:
- 5525269
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 3:2; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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Isolation and partial characterization of mutagen-sensitive and DNA repair mutants of Chinese hamster fibroblasts
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Journal Article
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Wed Dec 31 23:00:00 EST 1980
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·
OSTI ID:5306778
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Journal Article
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· Mol. Cell. Biol.; (United States)
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Journal Article
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Thu Dec 31 23:00:00 EST 1981
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
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OSTI ID:5615026
Related Subjects
550400 -- Genetics
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIGENS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CULTURES
DNA
DRUGS
ELECTROMAGNETIC RADIATION
GENE MUTATIONS
GENES
GLYCOSIDES
HAMSTERS
MAMMALS
MATERIALS
MUTAGENESIS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OUABAIN
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
STROPHANTHINS
TOXIC MATERIALS
TOXINS
ULTRAVIOLET RADIATION
VERTEBRATES
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIGENS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CULTURES
DNA
DRUGS
ELECTROMAGNETIC RADIATION
GENE MUTATIONS
GENES
GLYCOSIDES
HAMSTERS
MAMMALS
MATERIALS
MUTAGENESIS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OUABAIN
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
STROPHANTHINS
TOXIC MATERIALS
TOXINS
ULTRAVIOLET RADIATION
VERTEBRATES