DNA-mediated cotransfer of excision repair capacity and drug resistance into Chinese hamster ovary mutant cell line UV-135
The authors have investigated DNA-mediated transfer of aminopterin resistance conferred by plasmid and UV resistance conferred by genomic DNA to the Chinese hamster ovary (CHO) cell line UV-135, a UV-sensitive mutant defective in nucleotide excision repair. Plasmid pSV2gpt-CaPO/sub 4/ coprecipitates induced aminopterin resistance with equal efficiency in the 6-thioguanine-resistant, aminopterin-sensitive, repair-proficient parental line AA8-4(tg-1) and in UV-135(tg-2). Genetic and molecular evidence for genomic DNA-mediated transformation of UV-135(tg-2) cells with a putative excision repair gene were obtained. The overall frequency of drug and UV resistance cotransformation was 8 X 10(8) per cell plated. This frequency was ca. 200- to 500-fold greater than that expected from coincident but independent UVr reversion and plasmid gene transfer events. DNA transfer techniques with this CHO system will be useful for further analysis of the essential structural DNA sequences, gene cloning, and expression of functional excision repair genes.
- Research Organization:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- OSTI ID:
- 6305813
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 4:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CHO CELLS
DNA REPAIR
GENETICS
RADIOSENSITIVITY
AMINOPTERIN
DNA
MUTANTS
PLASMIDS
ULTRAVIOLET RADIATION
AMINES
ANIMAL CELLS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
CELL CONSTITUENTS
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PTERIDINES
RADIATIONS
RECOVERY
REPAIR
560121* - Radiation Effects on Cells- External Source- (-1987)
550400 - Genetics