Cellulase production based on hemicellulose hydrolysate from steam-pretreated willow
- Univ. of Lund (Sweden)
- Technical Univ., Budapest (Hungary)
The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good cellulose secreting micro-organism, using willow as the carbon source. The willow, which is a fast-growing energy crop in Sweden, was impregnated with 1-4% SO{sub 2} and steam-pretreated for 5 min at 206{degrees}C. The pretreated willow was washed and the wash water, which contains several soluble sugars from the hemicellulose, was supplemented with fibrous pretreated willow and used for enzyme production. In addition to sugars, the liquid contains degradation products such as acetic acid, furfural, and 5-hydroxy-methylfurfural, which are inhibitory for microorganisms. The results showed that 50% of the cellulose can be replaced with sugars from the wash water. The highest enzyme activity, 1.79 FPU/mL and yield, 133 FPU/g carbohydrate, was obtained at pH 6.0 using 20 g/L carbon source concentration. At lower pHs, a total lack of growth and enzyme production was observed, which probably could be explained by furfural inhibition. 15 refs., 5 figs., 4 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 576241
- Report Number(s):
- CONF-960539-; ISSN 0273-2289; TRN: 98:000980-0020
- Journal Information:
- Applied Biochemistry and Biotechnology, Vol. 63-65; Conference: 18. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 5-9 May 1996; Other Information: PBD: Spr 1997
- Country of Publication:
- United States
- Language:
- English
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