Effect of chemical treatments on the degradability of cotton straw by rumen microorganisms and by fungal cellulase

Journal Article · Tue Dec 01 04:00:00 EST 1981 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260231219· OSTI ID:5095073

Miron, J; Ben-Ghedalia, D

Three different chemical treatments-sulfur dioxide, ozone, and sodium hydroxide-were applied on cotton straw, and the effect on cell-wall degradability was assessed by using rumen microorganisms and Trichoderma reesei cellulase. Sulfur dioxide (applied at 70 degrees Celcius for 72 hours) did not change the lignin content of cotton straw but reduced the concentration of hemicellulose by 48%. Ozone exerted a dual effect, both on lignin (a 40% reduction) and hemicellulose (a 54% decrease). The treatment with NaOH did not solubilize cell-wall components. The in vitro organic matter digestibility with rumen fluid of cotton straw was increased significantly by ozone and SO/sub 2/ treatments, by 120% and 50%, respectively, but not by NaOH. T. reesei cellulase was applied on the chemically pretreated cotton straw at a low level (6 filter paper U/g straw, organic matter). The highest level of reducing sugars (30.6 g/100 g organic matter) was obtained with the O/sub 3/-cellulase combination, which solubilized 64% of the cellulose and 88% of the hemicellulose. The SO/sub 2/- and the NaOH-pretreated cotton straw were hydrolyzed by T. reesei cellulase to the same extent. The rumen fluid digestibility of the enzymatically hydrolyzed straw was not increased over that obtained with the chemical pretreatments. However, the fermentability of the combined treatments was increased markedly. In the O/sub 3/-cellulase-treated cotton straw, 83% of the rumen fluid digestible material consisted of highly fermentable components. Although ozone proved to be the most potent pretreatment for enzymic saccharification, the absolute result was modest. The limited effect of the combined O/sub 3/-cellulase treatment was probably associated with the pretreatment limitations, but not with the enzyme level. A hypothesis has been suggested as to the location of lignin and hemicellulose in the cell-wall unit of cotton straw. (Refs. 18).

• Research Organization: Agric. Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
• OSTI ID: 5095073
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 23:12; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
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59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALINE HYDROLYSIS
ANIMALS
BIODEGRADATION
BODY
CARBOHYDRATES
CELLULASE
CELLULOSE
CHALCOGENIDES
CHEMICAL REACTIONS
COTTON
DECOMPOSITION
DIGESTIVE SYSTEM
ENZYMATIC HYDROLYSIS
ENZYMES
FUNGI
GASTROINTESTINAL TRACT
GLYCOSYL HYDROLASES
HEMICELLULOSE
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
HYDROXIDES
IN VITRO
LIGNIN
LYSIS
MAMMALS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANS
OXIDATION
OXIDES
OXYGEN COMPOUNDS
OZONE
PLANTS
POLYSACCHARIDES
RUMINANTS
SACCHARIDES
SACCHARIFICATION
SODIUM COMPOUNDS
SODIUM HYDROXIDES
SOLVOLYSIS
STOMACH
STRAW
SULFUR COMPOUNDS
SULFUR DIOXIDE
SULFUR OXIDES
TRICHODERMA
TRICHODERMA VIRIDE
VERTEBRATES