Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pretreated sugarcane straw

Michelin, Michele; Ximenes, Eduardo; Polizeli, Maria T. M.; Ladisch, Michael R.

doi:10.1016/j.enzmictec.2023.110227

Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pretreated sugarcane straw

Journal Article · Fri Mar 10 00:00:00 EST 2023 · Enzyme and Microbial Technology

DOI:https://doi.org/10.1016/j.enzmictec.2023.110227· OSTI ID:1994479

Michelin, Michele ^[1]; Ximenes, Eduardo ^[2]; Polizeli, Maria T. M. ^[3]; Ladisch, Michael R. ^[4]

Purdue University, West Lafayette, IN (United States); Universidade de São Paulo (Brazil); University of Minho, Braga (Portugal); Purdue University
Indiana University, Bloomington, IN (United States)
Universidade de São Paulo (Brazil)
Purdue University, West Lafayette, IN (United States)

Relatively few studies have addressed the characterization of sugarcane straw (SCS) for production of fermentable sugars through enzyme hydrolysis. Straw is a major co-product of the sugarcane harvest in Brazil that has potential to sustainably increase cellulosic feedstocks in Brazil by 50%. Pretreatment of 10% w/v straw with liquid hot water (LHW) at 180°C for 50 min (severity, S of 4.05), solubilizes hemicellulose, preserves glucan, and generates 4.49 g/L soluble phenolic compounds in the resulting liquid. Extracts from washing pretreated solids with excess hot water followed by acetone resulted in 1.10 and 0.83 g/L phenolics, respectively. Here, acetone-derived extracts were more inhibitory and decreased glucose yield for enzyme hydrolysis of Solka Floc (a lignin-free cellulose) by 42%. In comparison, pretreated straw washed with hot water or acetone was readily hydrolyzed to 92% and 97% by cellulase enzyme. Hydrothermally treated SCS has the potential to provide a valuable and added source of fermentable sugars suitable for bioprocessing into biofuels and bioproducts when cellulase enzyme inhibitors are removed after pretreatment.

View Accepted Manuscript (DOE)

Research Organization:: Purdue Univ., West Lafayette, IN (United States); Purdue University, West Lafayette, IN (United States)

Sponsoring Organization:: Fundação de Amparo à Pesquisa do Estado de São Paulo; USDOE

Grant/Contract Number:: EE0008256; EE0008910

OSTI ID:: 1994479

Alternate ID(s):: OSTI ID: 2283509

Journal Information:: Enzyme and Microbial Technology, Journal Name: Enzyme and Microbial Technology Vol. 166; ISSN 0141-0229

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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