Stabilization and reutilization of Bacillus megaterium glucose dehydrogenase by immobilization
- Federal Univ. of Parana, Curitiba (Brazil)
- Oak Ridge National Lab., TN (United States)
Glucose dehydrogenase (GDH) from Bacillus megaterium was immobilized using aminopropyl controlled-pore silica (CPS, average pore sizes of 170 and 500 {angstrom}) as a support and glutaraldehyde as a bifunctional crosslinking agent. The CPS-immobilized enzyme could be reused 12 times and the best results were obtained using aminopropyl CPS-500 and bovine serum albumin as a feeder for stabilizing the protein layer on the support. DEAE-Sephadex (A-25 and A-50) was also used as a support for immobilizing GDH, with yields of around 42% for A-25 and 25-30% for A-50. The effect of pH on the immobilization procedure showed pH 6.5 to be better than pH 7.5 with respect to the recovery of enzyme activity. Both preparations of DEAE-Sephadex immobilized GDH could be reused several times and were thermostable at 400{degrees}C for 7 h. The kinetic parameters as Michaelis constant and maximum rate were determined for the immobilized enzyme and compared with those for the freeform. 9 refs., 6 figs., 3 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 576230
- Report Number(s):
- CONF-960539-; ISSN 0273-2289; TRN: 98:000980-0014
- Journal Information:
- Applied Biochemistry and Biotechnology, Vol. 63-65; Conference: 18. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 5-9 May 1996; Other Information: PBD: Spr 1997
- Country of Publication:
- United States
- Language:
- English
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