Purification and characterization of endo-xylanases from Aspergillus Niger. III. An enzyme of PL 365

Journal Article · Mon Apr 01 04:00:00 EST 1985 · Biotechnol. Bioeng.; (United States)

OSTI ID:5199351

Fournier, R A; Frederick, M M; Frederick, J R; Reilly, P J

An endo-xylanase (1,4-..beta..-D-xylan xylanohydrolase, EC 3.2.1.8) from Aspergillus niger was purified to homogeneity by chromatography with Ultrogel AcA 54, SP-Sephadex C-25 at pH 4.5, DEAE-Sephadex A-25 at pH 5.4, Sephadex G-50, and DEAE-Sephadex A-25 at pH 5.15. The enzyme was active on soluble xylan, on insoluble xylan only after arabinosyl-initiated branch points were removed, and on xylooligosaccharides longer than xylotetraose. There was slight activity on carboxymethyl-cellulose, arabinogalactan, glucomannan, and p-nitrophenyl-..beta..-D- glucopyranoside. The main products of the hydrolysis of soluble and insoluble xylan were oligosaccharides of intermediate length, especially the tri- and pentasaccharides. The isolectric point of the enzyme was 3.65. It had a molecular weight of 2.8 x 10/sup 4/ by SDS-gel electrophoresis, and was high in acidic amino acids but low in those containing sulfur. Highest activity in a 20-min assay at pH 5 was between 40 and 45 degrees C, with an activation energy up to 40 degrees C of 11.1 kJ/mol. The optimum pH for activity was at 5.0. The enzyme was strongly activated by Ca/sup 2 +/. 15 references.

• Research Organization: Iowa State Univ., Ames
• OSTI ID: 5199351
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:4; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ENERGY
ASPERGILLUS
CARBOHYDRATES
CHEMICAL PREPARATION
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
ENERGY
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HEMICELLULOSE
HYDROLASES
HYDROLYSIS
LYSIS
MOLECULAR WEIGHT
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PH VALUE
PLANTS
POLYSACCHARIDES
PURIFICATION
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
SYNTHESIS
XYLANASE
XYLANS