Production of 2,3-butanediol from pretreated corn cob by Klebsiella oxytoca in the presence of fungal cellulase

Journal Article · Wed Dec 31 04:00:00 EST 1997 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/BF02920419· OSTI ID:576219

Cao, Ningjun; Youkun, Xia; Tsao, G T ^[1]

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1. Purdue Univ., West Lafayette, IN (United States)

A simple and effective method of treatment of lignocellulosic material was used for the preparation of corn cob for the production of 2,3-butane-diol by Klebsiella oxytoca ATCC 8724 in a simultaneous saccharification and fermentation process. During the treatment, lignin, and alkaline extractives were solubilized and separated from cellulose and hemicellulose fractions by dilute ammonia (10%) steeping. Hemicellulose was then hydrolyzed by dilute hydrochloric acid (1%, w/v) hydrolysis at 100{degrees}C at atmospheric pressure and separated from cellulose fraction. The remaining solid, with 90% of cellulose, was then used as the substrate. A butane-diol concentration of 25 g/L and an ethanol concentration of 7 g/L were produced by K. oxytoca from 80 g/L of corn cob cellulose with a cellulose dosage of 8.5 IFPU/g corn cob cellulose after 72 h of SSF. With only dilute acid hydrolysis, a butanediol production rate of 0.21 g/L/h was obtained that is much lower than the case in which corn cob was treated with ammonia steeping prior to acid hydrolysis. The butanediol production rate for the latter was 0.36 g/L/h. 26 refs., 5 figs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 576219
• Report Number(s): CONF-960539--
• Journal Information: Applied Biochemistry and Biotechnology, Journal Name: Applied Biochemistry and Biotechnology Vol. 63-65; ISSN 0273-2289; ISSN ABIBDL
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ACID HYDROLYSIS
ALCOHOLS
AMMONIA
BIOMASS
BUTANE
CELLULASE
CELLULOSE
ETHANOL
FERMENTATION
HEMICELLULOSE
HYDROCHLORIC ACID
KLEBSIELLA
LIGNIN
MAIZE
SACCHARIFICATION