Improved alcohol production employing SSF with thermotolerant yeast
- Purdue Univ., West Lafayette, IN (United States)
Simultaneous saccharification and fermentation (SSF) involves the enzymatic hydrolysis of cellulose and the yeast fermentation of sugars to ethanol simultaneously in the same reactor. For the effective SSF process to produce ethanol from lignocellulose, it is required to remove the physical and chemical barrier around cellulose fibers and make cellulose more accessible to cellulose. Furthermore, it is preferred to have the compatible fermentation and saccharification conditions (e.g., temperature and pH). The process for pretreatment of lignocellulosic biomass involves the steeping in ammonia solution to remove lignin followed by dilute acid (1%, w/w) hydrolysis of hemicellulose fraction. The ammonia steeping removes over 70% of lignin and consequently facilitates the removal of hemicellulose by dilute acid. Dilute acid hydrolysis of hemicellulose yielding hydrolysate with sugar concentration of up to 8%. This fraction was used as substrate for ethanol production with xylose fermenting yeast strain. After lignin and hemicellulose were removed, the cellulose fraction was used as substrate in the SSF process for ethanol production. High yield of ethanol of over 60 g/L was produced by the thermotolerant yeast within 80 hours of SSF with a low enzyme loading of 8 IFPU/g cellulose.
- OSTI ID:
- 478657
- Report Number(s):
- CONF-960958--
- Country of Publication:
- United States
- Language:
- English
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