Somatostatin reduces sup 3 H-thymidine incorporation and c-myc, but not thyroglobulin ribonucleic acid levels in human thyroid follicular cells in vitro

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Journal of Clinical Endocrinology and Metabolism; (USA)

DOI:https://doi.org/10.1210/jcem-72-6-1364· OSTI ID:5761342

degli Uberti, E C; Hanau, S; Rossi, R; Piva, R; Margutti, A; Trasforini, G; Pansini, G; del Senno, L ^[1]

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1. Universita degli Studi di Ferrara, (Italy)

The action of somatostatin (SRIH) on {sup 3}H-thymidine (thy) incorporation and on c-myc and thyroglobulin RNA levels in a suspension of follicles from normal and goitrous human thyroid was examined. SRIH, at 10{sup {minus} 7} M concentration, inhibited basal thy incorporation (maximally by 4 h lasting for up 24 h), which effect was greater in goiter than in normal thyroid and was also detected in growing adherent epithelial cells. Moreover, in a follicle suspension SRIH prevented TSH-stimulated thy incorporation, both in normal and in goitrous thyroid. Basal expression of c-myc RNA was not affected by SRIH in either tissue, whereas the TSH-stimulated c-myc RNA level was significantly reduced in goiter. No effect of SRIH was observed on basal or TSH-stimulated thyroglobulin RNA levels. SRIH did not alter basal cAMP concentrations in normal or goitrous follicles, but it significantly reduced TSH-stimulated cAMP accumulation both in normal thyroid and in goiter. Overall, our data indicate a direct inhibitory action of SRIH on growth, but not on differentiation, of human thyroid, probably by a mechanism not entirely cAMP dependent.

• OSTI ID: 5761342
• Journal Information: Journal of Clinical Endocrinology and Metabolism; (USA), Journal Name: Journal of Clinical Endocrinology and Metabolism; (USA) Vol. 72:6; ISSN JCEMA; ISSN 0021-972X
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
DISEASES
ENDOCRINE DISEASES
GLOBULINS
GOITER
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MAN
METABOLISM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RNA
SOMATOSTATIN
SYNTHESIS
THYMIDINE
THYROGLOBULIN
THYROID CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES