Regulation of thyroid peroxidase activity by thyrotropin, epidermal growth factor and phorbol ester in porcine thyroid follicles cultured in suspension

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(89)90035-0· OSTI ID:5128234

Kasai, Kikuo; Hiraiwa, Masaki; Emoto, Tatsushi; Hattori, Yoshiyuki; Shimoda, Shin-Ichi ^[1]; Ohmori, Takeshi; Koizumi, Narumi; Hosoya, Toichiro ^[2]

---

1. Dokkyo Univ. School of Medicine, Tochigi (Japan)
2. Chiba Univ. (Japan)

The activity of thyroid peroxidase (TPO) in porcine follicles cultured for 96 h in suspension with five hormones (5H) still attained over 50% of that in the freshly isolated follicles. On the other hand, the activity in those cultured with 5H + TSH (6H) was several times higher than that cultured with 5H after 96 h, although an initial decrease of TPO activity during the first 24 h of culture was observed in both conditions. The ability of follicles to metabolize iodide when cultured with 6H for 96 h was also several times higher than that of those cultured with 5H. The half-maximal dose of TSH for stimulation of TPO activity and iodide metabolism was 0.03 - 0.04 mU/ml and the effect was mediated by cAMP. These results indicate that in porcine thyroid follicles in primary suspension culture, TPO activity as well as the ability of iodide metabolism is induced by chronic TSH stimulation. In addition, epidermal growth factor and phorbol 12-myristate 13-acetate completely inhibited TSH stimulation on both activities and also basal (5H) activity of iodide metabolism.

• OSTI ID: 5128234
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 45:16; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CARCINOGENS
CELL CULTURES
DOMESTIC ANIMALS
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
ESTERS
FUNCTIONS
GLANDS
GROWTH FACTORS
HALIDES
HALOGEN COMPOUNDS
HORMONES
INHIBITION
IODIDES
IODINE COMPOUNDS
MAMMALS
METABOLISM
MITOGENS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PEROXIDASES
PHORBOL ESTERS
PITUITARY HORMONES
PROTEINS
SWINE
THYROID
TSH
VERTEBRATES