Liposome kinetics in infarcted canine myocardium

Journal Article · · J. Cardiovasc. Pharmacol.; (United States)

DOI:https://doi.org/10.1097/00005344-198411000-00003· OSTI ID:5757284

Caride, V J; Twickler, J; Zaret, B L

To study the mechanisms and kinetics of liposome deposition in the region of the experimental myocardial infarction, the myocardial distribution of positive and negative liposomes was determined as a function of regional myocardial blood flow and time after administration. The study was performed in dogs at 1 and 24 h following experimental myocardial infarction. Twenty-four hours after coronary artery occlusion, the initial myocardial distribution of positive and negative liposomes (2 min) is directly proportional to regional myocardial blood flow. With time, there is reduction of the radiotracer associated with negative liposomes from all myocardial regions (p less than 0.01). In contrast, in areas of moderate and severe blood flow reduction, there is progressive accumulation of tracers entrapped or incorporated in positive liposomes. This increment becomes significant in 120 min (p less than 0.005). Similar findings are observed in studies performed 1 h after coronary artery occlusion. Dual-label liposomes ((/sup 3/H)cholesterol and (99mTc)diethylenetriamine pentaacetic acid) were used to study the integrity of liposomes in normal and ischemic myocardium. Significant dissociation of the aqueous and lipid labels of positive liposomes is observed 1 h following coronary artery occlusion. In the 24-h myocardial infarction model, dissociation of the aqueous and lipid labels in ischemic myocardium is also observed. This phenomenon is more pronounced with positive than with negative liposomes (p less than 0.02).

• Research Organization: Yale Univ. School of Medicine, New Haven, CT
• OSTI ID: 5757284
• Journal Information: J. Cardiovasc. Pharmacol.; (United States), Journal Name: J. Cardiovasc. Pharmacol.; (United States) Vol. 6:6; ISSN JCPCD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTERIES
BIOCHEMICAL REACTION KINETICS
BLOOD FLOW
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CORONARIES
DISEASES
DOGS
HEART
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPOSOMES
MAMMALS
MUSCLES
MYOCARDIAL INFARCTION
MYOCARDIUM
ORGANOIDS
ORGANS
REACTION KINETICS
RETENTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES