The recycling of a secretory granule membrane protein

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90249-8· OSTI ID:5745062

Hunter, A; Phillips, J H ^[1]

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1. Univ. of Edinburgh Medical School (United Kingdom)

The authors have used N-hydroxysuccinimido-d-biotin as a reagent for labeling proteins exposed at the surface of cultured bovine adrenal chromaffin cells during Ba{sup 2+}-stimulated secretion. A specific secretory granule membrane constituent, dopamine-{beta}-hydroxylase (DBH), has been investigated using immunoprecipitation followed by electrophoresis. Within 30 min of stimulation, exposed DBH had been cleared from the cell surface. Nevertheless, quantitation of labeled DBH using ({sup 125}I)streptavidin suggested that it remained undegraded over a period of 24 h, a time during which secretory granule stores of catecholamines were being replenished. Subcellular fractionation of the cultured cells suggested that, after 3 or 4 h, the biotinylated DBH, which was still membrane-bound, was located in particulate material that also contained cytochrome b{sub 561}, another major secretory granule membrane component.

• OSTI ID: 5745062
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 182:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINES
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZOLES
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOTIN
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATECHOLAMINES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DOPAMINE
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYLASES
IMIDAZOLES
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PHENOLS
PHOSPHORS
POLYPHENOLS
PROTEINS
RADIOISOTOPES
SODIUM COMPOUNDS
SODIUM IODIDES
SYMPATHOMIMETICS
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS