Nonreutilizaton of adrenal chromaffin granule membranes following secretion

Thesis/Dissertation · Mon Dec 31 23:00:00 EST 1984

OSTI ID:6942390

Nobiletti, J B

The intracellular postexocytotic fate of the adrenal chromaffin granule membrane (reutilization vs. nonreutilization) was addressed through two experimental approaches. First, (/sup 3/H) leucine pulse-chase labeling experiments were conducted in two systems - the isolated retrograde perfused cat adrenal gland and cultured bovine adrenal chromaffin cells to compare chromaffin granule soluble dopamine-B-hydroxylase (DBH) turnover (marker for granule soluble content turnover) to that of membrane-bound DBH (marker for granule membrane turnover). Experiments in cat adrenal glands showed that at all chase periods the granule distribution of radiolabeled DBH was in agreement with the DBH activity distribution (73% membrane-bound/27% soluble) - a result consistent with parallel turnover of soluble and membrane-bound DBH. Experiments in cultured bovine cells showed that labeled soluble and membrane-bound DBH had parallel turnover patterns and at all chase period, the distribution of radiolabeled DBH between the soluble contents and membranes was similar to the DBH activity distribution (50% soluble/50% membrane-bound). The above experiments showed that the soluble contents and membranes turnover in parallel and are consistent with nonreutilization of chromaffin granule membranes following exocytosis. Isolated retrograde perfused bovine adrenal glands were subjected to repetitive acetylcholine stimulation to induce exocytosis and then the dense and less-dense chromaffin granule fractions were isolated. Since both approaches gave results consistent with membrane nonreutilization, the authors conclude that once a chromaffin granule is involved in exocytosis, its membrane is not reutilized for the further synthesis, storage, and secretion of catecholamines.

• Research Organization: State Univ. of New York Downstate Medical Center, Brooklyn (USA). Dept. of Microbiology and Immunology
• OSTI ID: 6942390
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
AMINES
AMINO ACIDS
ANIMALS
AROMATICS
BIOLOGICAL LOCALIZATION
BODY
CARBOXYLIC ACIDS
CATECHOLAMINES
CATS
CATTLE
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
DOMESTIC ANIMALS
ENDOCRINE GLANDS
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
MEMBRANES
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PHENOLS
POLYPHENOLS
RUMINANTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES