Protein phosphorylation during 1-methyladenine-induced maturation of Asterias oocytes

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90269-3· OSTI ID:5745044

Haneji, Tatsuji; Koide, S S ^[1]

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1. Population Council, New York, NY (United States) Marine Biological Lab., Woods Hole, MA (United States)

Maturation was induced in Asterias oocytes with 1-methyladenine (1-MA) at a final concentration of 2 {mu}M. At 5, 10, and 30 min of treatment, oocytes were homogenized and the cytosolic fraction was prepared. The cytosol was incubated with ({gamma}-{sup 32}P)ATP and ({gamma}-{sup 32}P)GTP. The phosphorylated proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the radioactivity in the gels was determined by autoradiography. The cytosol prepared from 1-MA-treated oocytes incubated with ({gamma}-{sup 32}P)ATP showed a marked increase in the radiolabeling of proteins with estimated molecular weights of 70,000 and 62,000 Da. With ({gamma}-{sup 32}P)GTP a 56,000-Da protein showed increased radiolabeling. The present finding suggests that an early biochemical event of 1-MA-induced oocyte maturation in Asterias is the stimulation of phosphorylation of specific proteins.

• OSTI ID: 5745044
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 182:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
ATP
AUTORADIOGRAPHY
AZAARENES
CHEMICAL REACTIONS
DRUGS
ELECTROPHORESIS
GERM CELLS
HETEROCYCLIC COMPOUNDS
MATURATION
MEMBRANE PROTEINS
NUCLEOTIDES
OOCYTES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORYLATION
PROTEINS
PURINES
RECEPTORS