Protein phosphorylation during 5-hydroxytryptamine-induced maturation of Spisula oocytes

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90041-9· OSTI ID:5267237

Haneji, Tatsuji; Koide, S S ^[1]

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1. The Population Council, New York, NY (USA) The Marine Biological Laboratory, Woods Hole, MA (USA)

Maturation was induced in Spisula oocytes with 5-hydroxytryptamine (5-HT) creatinine sulfate at a final concentration of 5 {mu}M. After 10 and 30 min of treatment, oocytes were homogenized and the cytosolic and particulate fractions were prepared. The fractions were incubated with ({gamma}-{sup 32}P)GTP and ({gamma}-{sup 32}P)ATP. The phosphorylated proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The radioactivity in the gels was determined by autoradiography. With ({gamma}-{sup 32}P)GTP a marked increase in the radiolabeling of proteins with an estimated M{sub r} of 47,000 and 20,000 in the cytosolic and particulate fractions, respectively, was demonstrated with the 5-HT-treated oocytes, whereas no stimulation was demonstrable with the use of ({gamma}-{sup 32}P)ATP. A significant increase in GTP-mediated protein phosphorylation occurred within 10 min after 5-HT treatment before the occurrence of germinal vesicle breakdown, suggesting that this post-translation modification of proteins is an early action of the neurotransmitter in the induction of meiotic reinitiation in oocytes.

• OSTI ID: 5267237
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 177:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINES
AROMATICS
ATP
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
AZAARENES
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
GERM CELLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPES
LIGHT NUCLEI
MATURATION
MOLECULAR BIOLOGY
NEUROREGULATORS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ONTOGENESIS
OOCYTES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
POST-TRANSLATION MODIFICATION
PROTEINS
PYRROLES
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
SEROTONIN
SYMPATHOMIMETICS
TRYPTAMINES
ZYGOTES