Purothionin from wheat endosperm reversibly blocks myogenic differentiation of chick embryonic muscle cells in culture

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90409-6· OSTI ID:5745022

Kwak, Kyu Bong; Lee, Young Sup; Suh, Se Won; Ha, Doo Bong; Chung, Chin Ha ^[1]; Chung, Chin Sang ^[2]

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1. Seoul National Univ. (Korea)
2. Chungnam National Univ., Taejon (Korea)

Purothionin from wheat endosperm is a cysteine-rich, basic polypeptide of about 5,000 Da, which modifies membrane permeability of cultured mammalian cells. This peptide was found to block fusion of chick embryonic muscle cells in culture but allows proliferation and alignment. A purothionin concentration of 6 {mu}m/ml was necessary for the complete prevention of myotube formation. Under similar conditions, incorporation of ({sup 35}S) methionine occurred normally but the synthesis of muscle-specific proteins including creatin kinase and acetylcholine receptor was strongly inhibited. In addition, purothionin blocked the uptake of {sup 86}Rb{sup +}, immediately after its addition to the cultured myoblasts. These results suggest that purothionin exerts its regulatory effect on the transition from proliferative to differentiative myoblasts by interfering with membrane permeability or intercellular contact and recognition, which are necessary for the initiation of muscle differentiation.

• OSTI ID: 5745022
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 183:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIRDS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL DIFFERENTIATION
CELL MEMBRANES
CEREALS
CHICKENS
CREATINE
CYSTEINE
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EMBRYONIC CELLS
ENDOSPERM
ENZYMES
ESTERS
EVEN-ODD NUCLEI
FOWL
GRAMINEAE
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
LILIOPSIDA
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
MEMBRANE PROTEINS
MEMBRANES
METHIONINE
MINUTES LIVING RADIOISOTOPES
MUSCLES
MYOBLASTS
NEUROREGULATORS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASYMPATHOMIMETICS
PEPTIDES
PERMEABILITY
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PLANT TISSUES
PLANTS
POLYPEPTIDES
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RECEPTORS
RUBIDIUM 86
RUBIDIUM ISOTOP
SULFUR 35
SULFUR ISOTOPES
THIOLS
TISSUES
TRANSFERASES
UPTAKE
VERTEBRATES
WHEAT