Sonic Hedgehog signaling and Gli-1 during embryonic chick myogenesis

Journal Article · Sat Dec 15 00:00:00 EST 2018 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2018.11.071· OSTI ID:23134286

Teixeira, John Douglas; Andrade Rosa, Ivone de ^[1]; Brito, José ^[2]; Maia de Souza, Yuli Rodrigues; Machado, Marcelo Pelajo ^[3]; Costa, Manoel Luis; Mermelstein, Claudia ^[1]

---

1. Laboratório de Diferenciação Muscular e Citoesqueleto, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ (Brazil)
2. Laboratório de Proliferação e Diferenciação Celular, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ (Brazil)
3. Laboratório de Patologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ (Brazil)

Highlights: • Gli-1 is present in aggregates near the nuclei of chick myoblasts and myotubes. • Gli-1 aggregates colocalized with gamma-tubulin positive-centrosomes. • Recombinant Shh added to muscle cells induced the nuclear translocation of Gli-1. • Gli-1 aggregates are storage sites for its rapid cellular redistribution. The Sonic Hedgehog signaling (Shh) pathway has been implicated in both proliferation of myoblast cells and terminal differentiation of muscle fibers, and contradictory results of these effects have been described. To clarify the role of Shh during myogenesis, we decided to study the effects of recombinant Shh and the distribution of Gli-1 during in vitro and in situ embryonic chick skeletal muscle differentiation at later stages of development. Gli-1 was found in small aggregates near the nucleus in mononucleated myoblasts and in multinucleated myotubes both in vitro and in situ chick muscle cells. Some Gli-1 aggregates colocalized with gamma-tubulin positive-centrosomes. Gli-1 was also found in striations and at the subsarcolemmal membrane in muscle fibers in situ. Recombinant Shh added to in vitro grown muscle cells induced the nuclear translocation of Gli-1, as well as an increase in the number of myoblasts and in the number of nuclei within myotubes. We suggest that Gli-1 aggregates observed in chick muscle cells near the nuclei of myoblasts and myotubes could be a storage site for the rapid cellular redistribution of Gli-1 upon specific signals during muscle differentiation.

• OSTI ID: 23134286
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 507, Issue 1-4; Other Information: Copyright (c) 2018 The Authors. Published by Elsevier Inc.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
IN VITRO
MYOBLASTS
SIGNALS