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Sodium butyrate suppresses the transforming activity of an activated N-ras oncogene in human colon carcinoma cells

Journal Article · · Experimental Cell Research; (United States)
;  [1];  [2]
  1. Boston Univ., MA (United States)
  2. Dana Farber Cancer Inst., Boston, MA (United States)
+ Show Author Affiliations

The transforming activity of DNA from a newly established undifferentiated human colon carcinoma cell line (MIP-101) was tested in the NIH-3T3 transfection assay. Southern blot analysis of the transfectant DNA revealed the presence of a human N-ras oncogene. Here the authors report that there is a significant reduction in the transforming efficiency of the DNA from butyrate-treated MIP-101 cells. A nonspecific reduction in total DNA uptake as an explanation for these findings was eliminated by showing that there was similar uptake and expression of the thymidine kinase gene from the DNA of butyrate-treated and control MIP cells. An NIH-3T3 transformant carrying the human N-ras gene was evaluated for phenotypic reversion and DNA transforming ability after treatment with sodium butyrate. Although butyrate suppressed several transformed properties similar to MIP-101 cells, DNA from control and treated cultures had an identical level of transforming activity. The results suggest that the environment of the MIP cells may contain additional elements not present in the NIH-3T3 transformants which are required to observe the effect of butyrate on reduction of transforming activity.

OSTI ID:
5745015
Journal Information:
Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 184:1; ISSN ECREA; ISSN 0014-4827
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BUTYRIC ACID
CARBOXYLIC ACIDS
CARCINOMAS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
FIBROBLASTS
GENES
ISOTOPES
LIGHT NUCLEI
MOLECULAR BIOLOGY
MONOCARBOXYLIC ACIDS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENOTYPE
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SODIUM COMPOUNDS
SOMATIC CELLS
TUMOR CELLS