Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Activation of the H-ras oncogene in hepatocellular carcinomas initiated with diethylnitrosamine and promoted by a dietary methyl deficiency

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5202450
; ; ; ; ;

High molecular weight DNA was isolated from control livers and from hepatocellular carcinomas produced in F344 rats initiated with diethylnitrosamine (20 mg/kg body weight) then fed a methyl-deficient diet. The DNAs were used to transfect NIH 3T3 cells by the calcium phosphate precipitation technique. Cultures were scored for foci of morphologically transformed cells after 21 days. Three of 24 DNAs isolated from tumors initiated with diethylnitrosamine were able to transform NIH 3T3 cells (frequency = 0.01 to 0.04 foci/..mu..g DNA). Secondary transformants were produced when DNA isolated from transformed foci were used in the transfection assay. The presence of rat DNA sequences in the primary transformants was demonstrated using a probe specific for rat repetitive sequences. High molecular weight DNA isolated from each of the 3 primary transformants was digested with BamH1 and was subjected to Southern blot analysis using a /sup 32/P-labelled probe for the H-ras gene. Each transformant tested exhibited a fragment not present in 3T3 DNA that hybridized with the H-ras probe. Twelve control DNAs isolated from livers of tumor-free animals were negative in the transfection assay. The results are consistent with the involvement of the activated H-ras gene in the development of these chemically-initiated carcinomas in methyl-deficient animals.

Research Organization:
National Cancer Institute/FCRF and Program Resources, Inc., Frederick, MD
OSTI ID:
5202450
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

Similar Records

Activated neu oncogene sequences in primary tumors of the peripheral nervous system induced in rats by transplacental exposure to ethylnitrosourea
Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5230737
The effect of chemical carcinogens on DNA bypass replication and the development of in vitro and in vivo models for chemical mutagenesis
Thesis/Dissertation · Sat Dec 31 23:00:00 EST 1988 · OSTI ID:6905215
Activated protooncogenes in human lung tumors from smokers
Journal Article · Thu Feb 14 23:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:6161153

Related Subjects

550901 -- Pathology-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARCINOGENESIS
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DNA SEQUENCING
GENES
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER
LIVER CELLS
MAMMALS
MOLECULAR WEIGHT
NEOPLASMS
NITROSAMINES
NITROSO COMPOUNDS
NUCLEI
NUTRITIONAL DEFICIENCY
ODD-ODD NUCLEI
ONCOGENES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
VERTEBRATES