Activated protooncogenes in human lung tumors from smokers

Journal Article · Fri Feb 15 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.4.1085· OSTI ID:6161153

Reynolds, S H; Anna, C K; Brown, K C; Wiest, J S; Anderson, M W ^[1]; Beattie, E J ^[2]; Pero, R W ^[3]; Iglehart, J D ^[4]

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1. National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)
2. Beth Israel Medical Center, New York, NY (United States)
3. New York Univ., NY (United States)
4. Duke Univ., Durham, NC (United States)

Fourteen primary human lung tumor DNAs from smokers were analyzed for transforming activity by two DNA transfection assays. Activated protooncogenes were detected in 3 of 11 tumor DNAs by the NIK 3T3 focus assay, whereas activated protooncogenes were detected in 11 of 13 tumor DNAs by the NIH 3T3 cotransfection-nude mouse tumorigenicity assay. K- or NRAS genes activated by point mutation at codons 12 or 61 were detected in a large cell carcinoma, a squamous cell carcinoma, and 5 adenocarcinomas. An HRAS oncogene activated by a different mechanism was detected in an epidermoid carcinoma. One adenocarcinoma was found to contain an activated RAF gene. Two unidentified transforming genes were detected in a squamous cell carcinoma DNA and two adenocarcinoma DNAs. Eight of 10 lung adenocarcinomas that had formed metastases at the time of surgery were found to contain RAS oncogenes. No significant increase in metastasis was observed in the lung adenocarcinomas that contained one of more 6-kilobase EcoRI alleles of the LYMC gene. Overall, 12 of 14 (86%) of the lung tumor DNAs from smokers were found to contain activated protooncogenes. RAS oncogenes appear to play a role in the development of metastases in lung adenocarcinomas.

• OSTI ID: 6161153
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:4; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARCINOGENESIS
CARCINOMAS
CHEMICAL ACTIVATION
COLLOIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DISPERSIONS
DNA
DNA POLYMERASES
ENZYMES
EPIDEMIOLOGY
GENE AMPLIFICATION
GENES
ISOTOPES
LIGHT NUCLEI
LUNGS
MAMMALS
MAN
METASTASES
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ONCOGENES
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
RADIOISOTOPES
RESIDUES
RESPIRATORY SYSTEM
RFLPS
SMOKES
SOLS
TOBACCO SMOKES
TRANSFERASES
TUMOR CELLS
VERTEBRATES