Differentiation-linked expression of the plasminogen activator inhibitor type-2 gene in the human HL-60 promyelocytic cell line

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90316-9· OSTI ID:5744925

Arndt, A D; Gohill, J; Hart, D A; Rankin, K; Houwen, B ^[1]

---

1. Univ. of Calgary Health Sciences Centre, Alberta (Canada)

HL-60 is a human promyelocytic cell line which was found to be capable of differentiating toward a macrophage-like or granulocyte-like phenotype. Histochemical analysis demonstrated that incubation of cells in the presence of phorbol myristate acetate (PMA) or 1.25-dihydroxyvitamin D{sub 3} induced varying degrees of monocytic differentiation, while incubation in the presence of retinoic acid (RA) or dimethyl sulfoxide (DMSO) induced granulocytic differentiation. The differentiation induced by PMA, RA, and to a lesser extent DMSO, was accompanied by the induction of plasminogen activator inhibitor expression, mRNA analysis of control and PMA-induced cultures revealed the induction of a 2-kb message in treated cells which hybridized with a PAI-2-specific oligonucleotide probe. This is consistent with the literature concerning the expression of PAI by macrophages and granulocytes. Expression of PAI by cells of hematopoietic origin appears to be associated with differentiation of stimulation of committed cells.

• OSTI ID: 5744925
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 185:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CARBOXYLIC ACID ESTERS
CELL DIFFERENTIATION
COAGULANTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DMSO
DNA
DNA HYBRIDIZATION
DRUGS
ENZYME INHIBITORS
ESTERS
FIBRINOLYTIC AGENTS
GENE REGULATION
HEMATOLOGIC AGENTS
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
ISOTOPES
LEUKEMIA
LIGHT NUCLEI
MACROPHAGES
MAMMALS
MAN
MESSENGER-RNA
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHAGOCYTES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMINOGEN
PRIMATES
PROTEINS
RADIOISOTOPES
RETINOIC ACID
RNA
SOMATIC CELLS
SULFOXIDES
TUMOR CELLS
VERTEBRATES