Specific nuclear antigens expressed in differentiating human myeloid leukemia cells
The expression of three granulocyte-specific and two granulocyte-monocyte-specific nuclear antigens was detected by immunofluorescence with murine monoclonal antibodies in a series of human myeloid (HL-60, ML-2, KG-1, and B-III) and monocytic (THP-1) leukemia cells treated with chemical inducers of cell differentiation. Treatment of the promyelocytic HL-60 cells with dimethyl sulfoxide or retinoic acid induced the cells to acquire a granulocyte-like phenotype, while treatment of these cells with phorbol-12-myristate-13-acetate or 1,25-dihydroxyvitamin D/sub 3/ induced a phenotype that resembled that of monocytes-macrophages. The granulocyte-like phenotype was associated with the maintenance of one of the granulocyte-specific nuclear antigens and induction of the other four nuclear antigens. The monocytic-macrophage phenotype was associated with an increased expression of the granulocyte-monocyte-specific nuclear antigens but not of the granulocyte-specific antigens. The ML-2 and THP-1 cells were also susceptible to induction of cell differentiation and to changes in the expression of the nuclear antigens but to a lesser degree than were the HL-60 cells. The less differentiated KG-1 and B-II myeloid cells were either not responsive or responded only in a limited degree to the induction of cell differentiation or changes in the nuclear antigens. The authors suggest that the reactivity of cells with monoclonal antibodies to specific nuclear antigens can be used as a maturational marker in cell differentiation studies. Furthermore, nuclear antigens expressed early in cellular differentiation may provide information about changes in regulatory elements in normal and malignant cells.
- Research Organization:
- Argonne National Labs., IL
- OSTI ID:
- 6271394
- Journal Information:
- Proc. Am. Assoc. Cancer Res.; (United States), Vol. 25
- Country of Publication:
- United States
- Language:
- English
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