Gamma-ray-enhanced reactivation of irradiated adenovirus in Xeroderma pigmentosum and Cockayne syndrome fibroblasts
A ..gamma..-ray-enhanced reactivation (..gamma..RER) of uv-irradiated as well as of ..gamma..-irradiated human adenovirus type 2 (Ad 2) was detected following infection of normal, Xeroderma pigmentosum (XP), and Cockayne syndrome (CS) fibroblasts that had been preirradiated with ..gamma.. rays. Gamma-irradiated or nonirradiated fibroblasts were infected with either nonirradiated or irradiated Ad 2, and 48 hr after infection cells were examined for the presence of viral structural antigens (Vag) using immunofluorescent staining. Results obtained using seven different normal fibroblast strains showed that irradiation of host monolayers with 1 krad immediately prior to infection resulted in a ..gamma..RER factor +-SE of 4.5 +- 1.6 for uv-irradiated virus and 4.3 +- 1.3 for ..gamma..-irradiated virus. CS fibroblasts, as well as excision repair-deficient XP fibroblasts from complementation groups A and D, were all found to be capable of expressing ..gamma..RER of irradiated Ad 2. XP variant cells expressed lower levels of ..gamma..RER compared to most normal strains, suggesting a possible role for cellular postreplication repair in the mechanism responsible for ER in human cells. An excision-deficient XP fibroblast strain belonging to complementation group A, but derived from a patient afflicted with the severe De Sanctis-Cacchione form of XP, although proficient in ..gamma..RER of ..gamma..-irradiated Ad 2, yielded barely detectable levels of ..gamma..RER for uv-irradiated Ad 2.
- Research Organization:
- McMaster Univ., Hamilton, Ontario
- OSTI ID:
- 5742746
- Journal Information:
- Radiat. Res.; (United States), Vol. 94:3
- Country of Publication:
- United States
- Language:
- English
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FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
PHOTOREACTIVATION
RADIOINDUCTION
VIRUSES
XP CELLS
COBALT 60
EXPERIMENTAL DATA
EXTREME ULTRAVIOLET RADIATION
GAMMA RADIATION
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
ELECTROMAGNETIC RADIATION
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
PARASITES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
SOMATIC CELLS
ULTRAVIOLET RADIATION
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)