Failure of RNA synthesis to recover after UV irradiation: an early defect in cells from individuals with Cockayne's syndrome and xeroderma pigmentosum

Journal Article · Thu Apr 01 04:00:00 EST 1982 · Cancer Res.; (United States)

DOI:https://doi.org/10.1016/0027-5107(82)90047-1· OSTI ID:5440828

Mayne, L V; Lehmann, A R

Previous work has shown that in cells from the ultraviolet-sensitive genetic disorder, Cockayne's syndrome, DNA synthesis fails to recover after ultraviolet irradiation, despite the fact that these cells have no detectable defect in either excision or daughter-strand repair pathways. We now show that Cockayne cells, as well as cells from a number of patients with xeroderma pigmentosum, are sensitive to the lethal effects of UV irradiation in stationary phase under conditions in which no DNA is synthesized after irradiation. Furthermore, in normal and defective human fibroblasts, RNA synthesis is depressed after UV irradiation. In normal (dividing) cells, RNA synthesis recovers very rapidly, but this recovery does not occur in Cockayne cells, and it is reduced or absent in xeroderma pigmentosum cells from different complementation groups. Qualitatively, similar results are obtained with cells in stationary phase. The recovery of RNA synthesis in the various defective cell strains is not correlated with the overall extent of excision repair, but there is some correlation between recovery of RNA synthesis and cell survival after ultraviolet irradiation. These results implicate recovery of RNA synthesis as an important early response to ultraviolet irradiation.

• Research Organization: MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, Sussex, England
• OSTI ID: 5440828
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 42:4; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560114 -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
HEREDITARY DISEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
RADIATIONS
RECOVERY
REPAIR
RNA
SKIN DISEASES
SYNTHESIS
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM