Failure of RNA synthesis to recover after UV irradiation: an early defect in cells from individuals with Cockayne's syndrome and xeroderma pigmentosum
Previous work has shown that in cells from the ultraviolet-sensitive genetic disorder, Cockayne's syndrome, DNA synthesis fails to recover after ultraviolet irradiation, despite the fact that these cells have no detectable defect in either excision or daughter-strand repair pathways. We now show that Cockayne cells, as well as cells from a number of patients with xeroderma pigmentosum, are sensitive to the lethal effects of UV irradiation in stationary phase under conditions in which no DNA is synthesized after irradiation. Furthermore, in normal and defective human fibroblasts, RNA synthesis is depressed after UV irradiation. In normal (dividing) cells, RNA synthesis recovers very rapidly, but this recovery does not occur in Cockayne cells, and it is reduced or absent in xeroderma pigmentosum cells from different complementation groups. Qualitatively, similar results are obtained with cells in stationary phase. The recovery of RNA synthesis in the various defective cell strains is not correlated with the overall extent of excision repair, but there is some correlation between recovery of RNA synthesis and cell survival after ultraviolet irradiation. These results implicate recovery of RNA synthesis as an important early response to ultraviolet irradiation.
- Research Organization:
- MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, Sussex, England
- OSTI ID:
- 5440828
- Journal Information:
- Cancer Res.; (United States), Vol. 42:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Sensitivity of excision repair in normal human, xeroderma pigmentosum variant and Cockayne's syndrome fibroblasts to inhibition by cytosine arabinoside
Gamma-ray-enhanced reactivation of irradiated adenovirus in Xeroderma pigmentosum and Cockayne syndrome fibroblasts
Related Subjects
BIOLOGICAL REPAIR
BIOLOGICAL MODELS
RNA
BIOLOGICAL EFFECTS
BIOSYNTHESIS
DNA
HEREDITARY DISEASES
PATIENTS
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
BIOLOGICAL RECOVERY
DISEASES
ELECTROMAGNETIC RADIATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
SYNTHESIS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560114 - Radiation Effects on Biochemicals- In Animals- (-1987)