Ultrastructure and distribution of intracellular spicules in rat lung following chronic tobacco smoke exposure
Factors that contribute to the induction of lung tumors in F344 rats by chronic tobacco smoke exposure were studied. Previous studies had shown that tobacco smoke exposures of 1-2 years duration induced only one major type of lesion involving the respiratory airways, i.e., fibrotic and cellular enlargement of peribronchiolar alveolar septa. The airway epithelium in these areas was metaplastic and in some of the lesions, the airway lining epithelium advanced out onto the surfaces of adjacent alveoli. Epithelial cells in these lesions frequently contained elongated cytoplasmic inclusions which were oriented with their long dimensions roughly in the same plane as the long axis of the cell. Macrophages contained similar but larger inclusions. Because the composition of the inclusions could be indicative of their origin, the authors subjected samples of treated and control lung tissues concurrently to transmission electron microscopy and energy-dispersive X-ray fluorescence spectrometry. Spectra from inclusions of macrophages indicated the presence of the elements sulfur, phosphorus, aluminum, silicon, and iron. Spectra from type II cells, however, which did not contain inclusions, showed a different elemental composition. The results suggested that spicules were present in epithelial cells throughout the airways. Minor lesions corresponding to ''microinvasion'' of epithelium into the lamina propria and of capillaries into the epithelial layer were also found in the trachea.
- Research Organization:
- Bowling Green State Univ., OH
- OSTI ID:
- 5742580
- Journal Information:
- J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 74:3; ISSN JNCIA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
ALUMINIUM
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
CELL CONSTITUENTS
CHEMICAL COMPOSITION
CHRONIC EXPOSURE
COLLOIDS
CONNECTIVE TISSUE CELLS
CYTOPLASM
DISPERSIONS
ELECTRON MICROSCOPY
ELEMENTS
EMISSION SPECTROSCOPY
EPITHELIUM
FLUORESCENCE SPECTROSCOPY
HAZARDS
HEALTH HAZARDS
INJURIES
IRON
LUNGS
MACROPHAGES
MAMMALS
METALS
MICROSCOPY
MORPHOLOGICAL CHANGES
NONMETALS
ORGANS
PHAGOCYTES
PHOSPHORUS
RATS
RESIDUES
RESPIRATORY SYSTEM
RODENTS
SEMIMETALS
SILICON
SMOKES
SOLS
SOMATIC CELLS
SPECTROSCOPY
SULFUR
TISSUES
TOBACCO SMOKES
TOXICITY
TRACHEA
TRANSITION ELEMENTS
ULTRASTRUCTURAL CHANGES
VERTEBRATES