Evidence for serotonin binding in vitro by platelet factor 4 and beta-thromboglobulin
Journal Article
·
· Thromb. Res.; (United States)
Evidence is presented for in vitro high affinity binding of serotonin (5-HT) by beta-thromboglobulin (beta TG) and platelet factor 4 (PF4) from human blood. Results include: 1) identification by radioimmunoassay of PF4 in specifically bound material obtained by 5-HT affinity chromatography of human platelet extracts; 2) binding of 72% and 6% of radiolabelled PF4 on 5-HT and control affinity columns, respectively; and 3) binding of approximately 8 moles of 5-HT per mole of purified beta TG in the presence of ferrous ion and heparin in ultrafiltration studies, with Scatchard analysis indicating a dissociation constant of about 4 X 10(-8) M.
- Research Organization:
- School of Pharmaceutical Sciences, Rhodes University, Republic of South Africa
- OSTI ID:
- 5742494
- Journal Information:
- Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 29:3; ISSN THBRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CHEMICAL BONDS
COAGULANTS
DRUGS
GLOBULINS
GLOBULINS-BETA
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
MATERIALS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRROLES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOPROTECTIVE SUBSTANCES
SEROTONIN
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRYPTAMINES
59 BASIC BIOLOGICAL SCIENCES
AMINES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CHEMICAL BONDS
COAGULANTS
DRUGS
GLOBULINS
GLOBULINS-BETA
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
MATERIALS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRROLES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOPROTECTIVE SUBSTANCES
SEROTONIN
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRYPTAMINES