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Uptake and processing of human platelet factor 4 by hepatocytes

Journal Article · · Proc. Soc. Exp. Biol. Med.; (United States)
; ; ; ;

We previously demonstrated rapid clearance of human platelet factor 4 (PF4) from rabbit and rat blood, its accumulation in the liver, and elimination of PF4 degradation products in urine. The purpose of the present experiments was to characterize interaction of PF4 with cultured rat hepatocytes. /sup 125/I-PF4 was taken up by hepatocytes reaching maximum at 180 min. The association of /sup 125/I-PF4 with hepatocytes was two times greater at 37/sup 0/C than at 4/sup 0/C. At 37/sup 0/C degradation of /sup 125/-PF4 by hepatocytes was also observed as indicated by the increase of /sup 125/I-PF4 radioactivity soluble in 6% trichloroacetic acid. By contrast, no uptake of /sup 125/I-..beta..-thromboglobulin antigen was observed. Autoradiography demonstrated that short incubation (5-20 min) of /sup 125/I-PF4 with hepatocytes results in the association of /sup 125/I-radioactivity with cell membranes while after longer incubation (60 min) radioactivity was also localized in the endosomes. Heparin inhibited binding and uptake of /sup 125/I-PF4 radioactivity by hepatocytes. We propose that part of PF4 released in the circulating blood by activated platelets is bound to the surface of hepatocytes and that it is further processed by these cells.

Research Organization:
Temple Univ. School of Medicine, Philadelphia (USA)
OSTI ID:
5381185
Journal Information:
Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 186:3; ISSN PSEBA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HEMATOLOGIC AGENTS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIVER CELLS
MAMMALS
MEMBRANES
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
UPTAKE
VERTEBRATES