Effects of fluoride and ethane-1-hydroxy-1,1-diphosphonate on bone metabolism in the growing chick

Journal Article · Thu Jan 01 04:00:00 EST 1976 · J. Nutr., Suppl.; (United States)

OSTI ID:5742002

Chan, M M; Rucker, R B; Riggins, R S

White Leghorn cockerols were fed a semipurified diet (containing fluoride at 0 to 800 ppm) from the day of hatching. The birds were also injected daily with solutions containing ethane-1-hydroxy-1,1-diphosphonate (EHDP) at concentrations ranging from 5 to 20 mg as phosphorus/kg of body weight in isotonic saline. Changes in bone mineral compositions and plasma Ca, Mg and F were determined as well as alterations in bone pyrophosphatase and the amounts of bone cyclic-3',5'-adenosine monophosphate (cAMP). Since dietary fluoride in high amounts is known to stimulate new bone formation, the purpose of the study was to assess whether a potent inhibitor of mineralization, such as EHDP, might alter the response to high dietary fluoride. Although bone fluoride and magnesium were increased in relationship to dietary fluoride the administration of EHDP had little effect on these changes at low to moderate levels of fluoride. The levels of soluble bone pyrophosphatase were not greatly influenced by changes in dietary fluoride. Administration of EHDP at 20 mg P/kg, however, typically decreased the levels of bone pyrophosphatase. Bone cAMP did not appear to be influenced by either dietary fluoride or EHDP. Using tissue culture techniques, the effects of fluoride on calcium uptake and release from embryonic chick bone were also studied. The presence of 1 mM fluoride in the medium appeared to stimulate calcium uptake and reduced calcium release from chick bone. In general, the results were in keeping with previous suggestions that the major effect of fluoride on bone is the formation of fluoroapatite. 40 references, 1 figure, 6 tables.

• Research Organization: Univ. of California, Davis
• OSTI ID: 5742002
• Journal Information: J. Nutr., Suppl.; (United States), Journal Name: J. Nutr., Suppl.; (United States) Vol. 106:6; ISSN JNSUA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BIRDS
BODY
BONE TISSUES
CALCIUM
CHEMICAL COMPOSITION
CHICKENS
CONNECTIVE TISSUE
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
FLUORIDES
FLUORINE COMPOUNDS
FOWL
HALIDES
HALOGEN COMPOUNDS
INGESTION
INHIBITION
INTAKE
METALS
MINERALIZATION
ORGANIC COMPOUNDS
ORGANS
RESPONSE MODIFYING FACTORS
SKELETON
STIMULATION
SYNTHESIS
TISSUES
UPTAKE
VERTEBRATES