Effects of nitrous oxide on oxygen consumption by isolated cerebral cortex mitochondria

Journal Article · Tue Apr 01 04:00:00 EST 1986 · Anesth. Analg. (Cleveland); (United States)

DOI:https://doi.org/10.1213/00000539-198604000-00008· OSTI ID:5737066

Becker, G L; Pelligrino, D A; Miletich, D J; Albrecht, R F

The influence of N2O on O2 consumption by mitochondria isolated from the cerebral cortex of goats was examined in incubations preequilibrated with N2O-O2 or N2-O2. Rates of O2 consumption were measured polarographically in a closed system while adenosine triphosphate (ATP) formation was maximal (after addition of excess adenosine diphosphate (ADP), state 3 respiration) and then when it was at zero (after addition of excess oligomycin, state 4 respiration). Compared with 90% N2, 90% N2O produced no change in the rate of state 3 respiration; but an observed 9% decrease in the state 4 rate and an 11% increase in the state 3: state 4 ratio were statistically significant (P less than 0.05). These differences were not seen with N2 and N2O at 70% rather than at 90%, or when succinate rather than pyruvate-malate was used as the respiratory substrate. We conclude the following: Unlike other inhalation anesthetics, N2O at comparable anesthetic concentrations does not inhibit mitochondrial electron transport or ATP formation coupled to it (oxidative phosphorylation). N2O does inhibit one or more other processes, as yet unidentified, which are energetically coupled to electron transport. The increased cerebral O2 consumption that accompanies N2O anesthesia cannot be attributed to a direct effect of N2O on mitochondrial respiration.

• Research Organization: Michael Reese Hospital and Medical Center, Chicago, IL
• OSTI ID: 5737066
• Journal Information: Anesth. Analg. (Cleveland); (United States), Journal Name: Anesth. Analg. (Cleveland); (United States) Vol. 4; ISSN AACRA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BODY
BRAIN
CALCIUM
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CEREBRAL CORTEX
CEREBRUM
CHALCOGENIDES
DOMESTIC ANIMALS
ELECTRON TRANSFER
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
GOATS
HADRONS
IN VITRO
INHIBITION
MAMMALS
METALS
MITOCHONDRIA
NERVOUS SYSTEM
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
NONMETALS
NUCLEONS
ORGANOIDS
ORGANS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
PROTONS
RESPIRATION
RUMINANTS
VERTEBRATES