Hemoglobin variants as models for investigation of dissociation of intact polypeptide chains by ESI tandem mass spectrometry
Conference
·
OSTI ID:5736827
Electrospray ionization mass spectroscopy (ESI-MS) is rapidly becoming a practical biochemical tool for peptide and protein sequence analysis. The utility of ESI-MS is through use of Collisionally Activated Dissociation (ESI-CAD-MS). Human hemoglobin (Hb, {approximately}62 kDa) consists of four polypeptide chains and a prosthetic heme group. There are over 400 Hb variants, characterized by amino acid substitutions in either the alpha or beta polypeptide chains. We investigated ESI-CAD-MS as a tool for rapidly analyzing amino acid substitutions, using eight Hb beta chain variants. The approximate location of the modification can be deduced from comparison of the CAD mass spectra and observance of the mass shifts of the fragment ion containing the substitution. Fragmentation occurs preferentially at the amino terminus of proline residues. For most substitutions, differences in CAD mass spectra were not seen. 2 figs.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (USA)
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 5736827
- Report Number(s):
- PNL-SA-19121; CONF-9105181--29; ON: DE91015265
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
AZOLES
BIOLOGICAL VARIABILITY
CARBOXYLIC ACIDS
CHARGED PARTICLES
DISSOCIATION
FRAGMENTATION
GLOBINS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZATION
IONS
MASS SPECTRA
MASS SPECTROSCOPY
MEASURING INSTRUMENTS
MEASURING METHODS
MOLECULAR IONS
MOLECULAR STRUCTURE
MUTANTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PIGMENTS
POLYPEPTIDES
PORPHYRINS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
SPECTRA
SPECTROSCOPY
400102 -- Chemical & Spectral Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
AZOLES
BIOLOGICAL VARIABILITY
CARBOXYLIC ACIDS
CHARGED PARTICLES
DISSOCIATION
FRAGMENTATION
GLOBINS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZATION
IONS
MASS SPECTRA
MASS SPECTROSCOPY
MEASURING INSTRUMENTS
MEASURING METHODS
MOLECULAR IONS
MOLECULAR STRUCTURE
MUTANTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PIGMENTS
POLYPEPTIDES
PORPHYRINS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
SPECTRA
SPECTROSCOPY