Effect of sodium selenite upon bromobenzene toxicity in rats. II. Metabolism

Journal Article · Tue Apr 01 04:00:00 EST 1986 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(86)90305-4· OSTI ID:5736411

Merrick, B A; Davies, M H; Schnell, R C

The effects of sodium selenite (12.5 or 30 mumol/kg, ip) upon bromobenzene metabolism were examined in male rats treated with selenite at 72 hr prior to bromobenzene exposure (7.5 mmol/kg, ip). The inhibitory nature of selenium treatment upon xenobiotic metabolism and increased production of hepatic thiol suggested that selenite might affect metabolic activation and detoxification of bromobenzene. Selenite treatment lowered in vivo covalent binding of (/sup 14/C)bromobenzene while the in vitro covalent binding of (/sup 14/C)bromobenzene in microsomes isolated from selenite-treated rats was unaffected compared to control. When the rate of (/sup 14/C)bromobenzene decline in whole blood was evaluated in selenite-treated rats over 48 hr after bromobenzene administration, no significant differences were observed when compared to control. Furthermore, values of glutathione conjugates and hydroxylated metabolites of bromobenzene were similar in urine samples collected over 48 hr from selenite and control rats. Mechanistically, reduction of bromobenzene hepatotoxicity by selenite is not mediated through an altered metabolism of bromobenzene, but by alteration of cellular events occurring after metabolic activation.

• Research Organization: Univ. of Nebraska Medical Center, Omaha
• OSTI ID: 5736411
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 2; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

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560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZENE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL HALF-LIFE
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY
BODY FLUIDS
COVALENCE
DIGESTIVE SYSTEM
ELEMENTS
GLANDS
HYDROCARBONS
KINETICS
LIVER
MAMMALS
MATERIALS
METABOLIC ACTIVATION
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
ORGANS
RATS
REACTION KINETICS
RODENTS
SELENIUM
SEMIMETALS
TOXICITY
URINE
VERTEBRATES
WASTES