Methods for human and murine sperm assays

Conference · Tue Jan 01 04:00:00 EST 1980

OSTI ID:5734267

Wyrobek, A J

Human sperm assays in the context of an epidemiological study provide an effective means to identify agents that affect testicular function. Four assays for human sperm anomaly (counts, motility, morphology, and double-fluorescence bodies) have been successfully used for a variety of human occupational and drug-related exposures. However, more research is needed to validate the genetic implications of sperm defects induced by exposure to chemicals in vivo. The F/sub 0/ and F/sub 1/ mouse-sperm assay, especially for induced morphological defects, provides a method of screening agents that may be potentially harmful to man and provides a means to assess the antifertility effects and consequences of induced sperm defect.

• Research Organization: California Univ., Livermore (USA). Lawrence Livermore Lab.
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5734267
• Report Number(s): UCRL-83854; CONF-800113-1
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
551000 -- Physiological Systems
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
560306 -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
FLUORESCENCE
GAMETES
GERM CELLS
LUMINESCENCE
MAMMALS
MAN
MICE
MORPHOLOGICAL CHANGES
MUTAGEN SCREENING
PRIMATES
QUANTITY RATIO
RISK ASSESSMENT
RODENTS
SCREENING
SPERMATOZOA
TOXIC MATERIALS
VERTEBRATES