Convenient method of establishing permanent lines of xeroderma pigmentosum cells. [Ultraviolet radiation]
Nine lymphoblastoid cell lines were established after transformation by Epstein-Barr virus of peripheral lymphocytes from four xeroderma pigmentosum (XP) patients, the parents of one XP patient, and three normal donors. All these cell lines proliferate as suspension in Roswell Park Memorial Institute Medium 1640 supplemented with 20% fetal bovine serum, without detectable release of infectious Epstein-Barr virus. Some characteristics of these cell lines, such as growth rates, chromosome numbers, uv sensitivities, and activities of unscheduled DNA syntheses induced by uv, 4-nitroquinoline 1-oxide, and N-methyl-N'-nitro-N-nitrosoguanidine, were determined. Results confirm that the properties related to XP are not altered by transformation with Epstein-Barr virus and are the same in degrees of defect as are those of dermal fibroblasts from the respective individuals. These XP and normal lymphoblastoid cell lines should be especially useful for biochemical studies on the mechanism of DNA repair, because they are easy to grow in mass culture.
- Research Organization:
- Tohoku Univ., Sendai, Japan
- OSTI ID:
- 5733296
- Journal Information:
- Cancer Res.; (United States), Vol. 38:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL PROLIFERATION
RADIOSENSITIVITY
CHROMOSOMAL ABERRATIONS
DNA REPLICATION
XP CELLS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
COMPARATIVE EVALUATIONS
DRUGS
ONCOGENIC VIRUSES
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
ANIMAL CELLS
BIOLOGICAL EFFECTS
DISEASES
ELECTROMAGNETIC RADIATION
MICROORGANISMS
MUTATIONS
NUCLEIC ACID REPLICATION
PARASITES
RADIATION EFFECTS
RADIATIONS
SKIN DISEASES
VIRUSES
560121* - Radiation Effects on Cells- External Source- (-1987)